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表皮生长因子 人类?????? SIGMA?E9644

recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
  • 亚博体育网上注册编号:E9644
  • 中文名称:表皮生长因子 人类
  • 英文名称:Epidermal Growth Factor human-EGF human
  • 英文别名:EGF LONG® EGF
  • CAS号:62253-63-8
  • 纯度:≥97% (SDS-PAGE)
  • 保存温度:−20 °C
  • Components

    Human EGF (hEGF) is an identical molecule to β-urogastrone, a molecule isolated on the basis of its ability to inhibit gastric acid secretion. EGF is structurally homologous to human transforming growth factor-α, and both exert their actions through EGF receptors. E9644 is the N-terminal methionyl form of natural mature EGF.

    Analysis Note

    The biological activity of this product is measured in a proliferation assay using BALB/MK cells. The EC50?is defined as the effective concentration of growth factor that elicits a 50% increase in cell growth in a cell based bioassay.

    Biochem/physiol Actions

    Epidermal Growth Factor (EGF) is a small mitogenic polypeptide present throughout a large number of tissues and body fluids in many mammalian species. EGF is a member of a growth factor family characterized by 6 conserved cysteine motifs that form three disulfide bonds. EGF is mitogenic for a variety of epidermal and epithelial cells, including fibroblasts, glial cells, vascular and corneal endothelial cells, bovine granulosa, HeLa cells, SV40-3T3 cells and mammary epithelial cells.?
    Cellular functions affected by EGF: mitosis, ion flux, glucose transport, glycolysis, nucleic acid and protein synthesis, survival, growth, proliferation and differentiation.
    Biological affects of EGF: inhibition of gastric acid secretion, fetal growth and development and neuromodulation in the central nervous system.?
    Pathways affected by EGF: EGFR signaling, MAPK cascade, PIP, Ca+2 signaling

    Caution

    This product should be stored at -20°C and will retain activity for two years. After reconstitution, it can be stored at 2-8°C for one month, or frozen in aliquots at -70°C or -20°C for longer periods.

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